INVISORB BLOOD UNIVERSAL KIT

   Overview

The Invisorb® Blood Universal Kit enables isolation of genomic DNA from variable amounts (1-10 ml) of whole blood or from corresponding amounts of buffy coat. Fresh or frozen whole blood treated with EDTA or citrate, but not with heparin, from common blood collection systems can be used.

   Benefits

• Easy handling and purification in a single tube, no risk of sample mix-up

• High flexibility

• Reduction of plastic consumption

• For In Vitro Diagnostic Use (CE-IVD)*

Product Characteristics

   Workflow

The kit uses a special lysis reagent for selective lysis of erythrocytes. After all erythrocyte components are removed, lymphocytes DNA can be isolated free of any interfering hemoglobin. The remaining lymphocytes are lysed with optimized buffer and proteins are digested with Proteinase K. DNA is precipitated by addition of isopropanol, recovered by centrifugation, washed and dried. Finally the DNA is resuspended in elution buffer. The concentration of the resulting DNA can be adapted to the needs of the user. The isolated DNA is highly chromosomal and well suitable for archiving. Due to the high purity, the isolated genomic DNA is ready to use for  downstream applications or can be stored at –20°C:

• PCR, real-time PCR

• RFLP analysis

• restriction enzyme digestion

• cloning

• sequencing

• STR and SNP analysis

• *) Compliance with EU Directive 98/79/EC on in vitro medical devices. Not for in vitro diagnostic use in countries where the EU Directive 98/79/EC on in vitro medical devices is not recognized.

Manual

MSDS

References