MSB Spin PCRapace
Options
250 or 500 Preparations
Overview
With about 7 min of processing time the MSB® Spin PCRapace is one of the fastest systems for the separation of PCR products from dNTPs, primers, enzymes, dye terminators, additives and salts. The kit is also a powerful and efficient tool for the concentration of PCR products. The MSB technology requires only two simple process steps: DNA binding to a membrane achieved in extremely low salt concentrations and the elution of DNA fragments directly after binding.
Benefits
- Pure PCR products in 7 minutes - only binding and elution
- Elution volume as little as 10 μl, also suitable for sample concentration
- Dye removal from Cycle-Sequencing reactions
Product Characteristics
Starting Material | Average Yield | Preparation Time |
up to 100 μl PCR-, Cycle Sequencing- cDNA synthesis-, ligation- and restriction digestion reaction mixture | up to 95 % of PCR products for fragment sizes from 80 bp - 30 kb | approx. 7 min |
Workflow
After adjustment of binding conditions the DNA fragments bind to the membrane of the spin filter. The flow-through contains all contaminants. The DNA fragments are eluted in a flexible volume of Elution Buffer or water. The recovery rate is nearly independent from PCR additives, PCR reaction volume and fragment sizes. The purified PCR product can be used in subsequent downstream applications:
- sequencing
- cloning
- ligation
- labelling
- digestion with restriction enzymes
- PCR
- Product: MSB Spin PCRapace
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- Availability: In Stock
Available Options
Tags: MSB Spin PCRapace