PSP SalivaGene DNA Kit

Options

50 or 250 Purifications 

Overview

The PSP® SalivaGene DNA Kit is designed for isolation of genomic, mitochondrial and bacterial DNA from SalivaGene stabilized samples.

Benefits

• Spin filter DNA extraction kit for use in combination with the SalivaGene sample collection sets (SalivaGene Collector, SalivaGene Collection Module II, SalivaGene Buccal Swab)

• High DNA yields from saliva samples without degradation

• High quality DNA from saliva performs as well as blood-derived DNA on downstream applications

• For In Vitro Diagnostic Use (CE-IVD)*

Product Characteristics

Workflow

The SalivaGene DNA stabilization buffer effects inactivation of DNases and prevents degradation of DNA, it preserves the microorganism titre and prelyses bacteria. The stabilized sample may be shipped in the tubes during 12 month at ambient temperature. The sample may be stored in the SalivaGene buffer at -20° for a several years. For the DNA extraction an aliquot may be used, the residual sample is stored for further extractions.

500 µl of the stabilized sample are used in the DNA isolation procedure. First the proteins are degraded with Proteinase K, followed by DNA-binding onto the membrane of the spin filter while contaminants pass though. After washing steps the DNA is eluted in either water or Elution Buffer provided with the kit. The purified, high-quality DNA is ready to use for subsequent downstream applications:

• PCR application

• genetic typing

• SNP and mutation analysis

• forensic analysis (e.g. screenings, paternity tests)

• pharmacogenomics

*) Compliance with EU Directive 98/79/EC on in vitro medical devices (Not for in vitro diagnostic use in countries where the EU Directive 98/79/EC on in vitro medical devices is not recognized.)

User Manual