Overview

The InviMag® Blood DNA Maxi Kit/ IG enables fully automated, true walk-away purification of genomic DNA from human blood samples on the InviGenius PLUS with sample volumes of up to 2 ml. Fresh or frozen whole blood treated with EDTA or citrate, but not with heparin, from common blood collection systems can be used. Different primary tubes can be placed directly into the sample loading rack. A defined and optimized protocol provides high yields of pure DNA. Automatic barcode reading enables full tracking of samples and reagents.

Benefits

• Ultra-pure genomic DNA for enhanced performance in sensitive downstream applications

• Fully automated, walk-away purification

• High reproducibility through standardized processing

• Barcodes enable tracking of samples and reagents

Product Characteristics

Workflow

The samples and reagents are placed in the loading rack and are identified by the automatic barcode scanner. Sample, reagents, and disposables set-up is software-supported.

The sample lysis is performed in an optimized lysis buffer directly on the InviGenius (PLUS) in the heat incubator for efficient sample lysis of up to 90 °C. The heated lid prevents condensation and aerosol formation.
The purified genomic DNA is of high quality and can be used for subsequent downstream applications:

The InviGenius uses magnetic rods to transport the DNA-binding magnetic particles through the various extraction phases: Binding-Washing-Elution. The subsequent steps after lysis, such as binding of the nucleic acid onto the InviMag beads, washing and final DNA elution are automatically performed in a fast and reproducible manner. No user intervention is necessary from sample loading to extracted DNA.

• PCR and real-time PCR

• genotyping, STR, and SNP analysis

• sequencing

Manual

MSDS