Monoclonal Mouse Antibody
Melanoma Cocktail 
F, P/H

Catalog No.: Mob 428 Concentrated 1ml
Mob 428-05 Concentrated 0.5ml
PDM 146 Prediluted 6ml

Immunogen: Pigmented melanoma metastases from lymph nodes (HMB45). Recombinant human MART-1 protein (A103). Recombinant tyrosinase protein T311.
Clone: HMB45+A103+T311
Isotype: HMB45 and A103, IgG1; T311, IgG2a
Positive Control: Metastatic melanoma in lymph node
Cellular Localization: Cytoplasmic
Specificity: The HMB45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas. Clone A103 recognizes a protein of 20 kDa, identified as MART-1 (melanoma antigen recognized by T cells-1) or Melan-A. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions.
Studies have also shown that MART-1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown tyrosinase to be a more sensitive marker when compared to HMB45 and MART-1. It has been shown to label a higher percentage of desmoplastic melanomas than HMB45. The combination of HMB45, MART-1 and tyrosinase make this triple antibody cocktail a first-order pan melanoma screener.

F=Frozen P=Formalin Fixed Paraffin Embedded H=Heat Induced Pretreatment E=Enzyme Pretreatment

For more information please visit the DBS website at dbiosys.com

Additional Information