Overview

The InviGene® Bisulfite Conversion Kit enables rapid and reliable bisulfite treatment and conversion of cfDNA and genomic DNA from whole blood, cell cultures, tissue samples and FFPE slides for methylation analysis.

Benefits

• Convenient procedure for fast and reliable results
• Fast method for complete bisulfite conversion of DNA for methylation analysis
• Isothermal protocol - flexible for use in heating blocks or all commonly used thermocylers
• Denaturation, conversion and purification combined in a single work process
• High recovery rates of ready-to-use DNA in small elution volume

Product Characteristics

Workflow

The InviGene Bisulfite Conversion Kit procedure comprises following steps:

• Denaturation and bisulfite conversion

• Binding of the DNA to the filter membrane and desulfonation

• Washing the filter bound DNA and elimination of alcohol

• Elution of DNA

The eluted DNA can be used in all downstream applications, including methylation-specific PCR, real-time PCR analysis, bisulfite sequencing etc

Application

Manual

MSDS