INVIMAG BLOOD DNA MINI KIT/ KFDUO

  • INVIMAG BLOOD DNA MINI KIT/ KFDUO

Overview

The InviMag® Blood DNA Mini Kit/ KFDuo allows for rapid and economical purification of genomic DNA directly from up to 200 µl of whole blood, buffy coat, bone marrow, and swabs using magnetic beads. The InviMag Blood DNA Mini Kit/ KFDuo is designed for semi-automated preparation on the KingFisher Duo workstation.

Benefits

  • Automated purification for reproducible high yields
  • No additional erythrocyte lysis
  • No cross contamination

Product Characteristics

Starting Material

Average Yield

Preparation Time

up to 200 µl of human or other mammalian blood (EDTA, citrate), rinse liquid from swabs

up to 30 µl buffy coat 
up to 20 µl bone marrow
up to 25 µl of non-mammalian blood

2 - 6 µg

about 60 min

Workflow

Beside the pre-filling of all cavities, all processing steps are performed by the robot. The blood sample is lysed in an optimized lysis buffer containing Proteinase K. The DNA binds to magnetic particles, followed by washing steps and the final elution.

The purified high quality DNA is ready to use for subsequent downstream applications or can be stored at –20 °C for subsequent use:

  • PCR
  • real-time PCR
  • RFLP analysis
  • genotyping
  • restriction enzyme digestion
  • hybridization
  • SNP analysis


Manual

MSDS

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  • Product: INVIMAG BLOOD DNA MINI KIT/ KFDUO
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