Overview

The InviMag® Blood DNA Mini Kit/ KFmL allows for rapid and economical purification of genomic DNA directly from up to 200 µl of whole blood, buffy coat, liquor, and bone marrow using magnetic beads. The InviMag Blood DNA Mini Kit/ KFmL is designed for semi-automated preparation on the KingFisher mL workstation.

Benefits

• Standardized processing ensures reproducible results

• No cross contamination

• For In Vitro Diagnostic Use (CE-IVD)*

Product Characteristics

The kits are also available without the KingFisher plastic.

Workflow

Beside the pre-filling of all cavities, all processing steps are performed by the robot. The blood sample is lysed in an optimized lysis buffer containing Proteinase K. The lysis step can be carried out direct on the platform at ambient temperature or outside on a thermomixer at 56°C while continuously shaking. In this case, the lysed samples are transferred onto the workstation to the subsequent automatic purification procedure based on magnetic beads. The DNA binds to magnetic particles, followed by washing steps and the final elution.

The purified DNA is ready to use for subsequent downstream applications or can be stored at –20 °C for subsequent use:

• PCR

• real-time PCR

• RFLP analysis

• genotyping

• restriction enzyme digestion

• hybridization

• SNP analysis

*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.

Application

Manual

MSDS

References