INVIMAG UNIVERSAL KIT/ STARLET

  • INVIMAG UNIVERSAL KIT/ STARLET

Overview

The InviMag® Universal Kit/ STARlet is designed for fully automated, magnetic bead based extraction of viral DNA/ RNA, genomic DNA, bacterial DNA from 96 samples per run with the STARlet system from Hamilton.

Benefits

  • One kit for a variety of starting materials and isolation of different nucleic acids under uniform conditions
  • Automated, walk-away DNA purification of 96 samples using magnetic beads
  • High reproducibility through standardized processing

Product Characteristics

Starting Material

Average Yield

Preparation Time

200 µl fresh or frozen blood*,

200 µl fresh or frozen plasma*/ serum
200 µl cell-free body fluids (urine)
200 µl rinse liquid from swab
200 µl transport media (Surepath, Thinprep)
200 µl supernatant from stool suspension
200 µl from liquid sputum, BAL, tracheal secrete
*) only EDTA/ Citrate stabilized

depending on starting material

approx. 180 min

Workflow

The STARlet instrument uses an 8-channel pipettor to transfer samples and buffers and a magnetic tool to separate the DNA/ RNA bound to magnetic particles during the various extraction phases: lysis, binding, washing and elution. The automated purification process results in a fast, reliable and robust technique.

After sample specific lysis, binding conditions are adjusted upon addition of Binding Solution. The genomic DNA and/or viral DNA/ RNA bind to the added magnetic particles and is separated from the solution by magnetic tool controlled by the STARlet system. Subsequent to three washing steps of the particle bound nucleic acids, the nucleic acids are finally eluted in Elution Buffer. 
Due to the high purity the eluted nucleic acids are ready-to-use use for subsequent downstream applications:

  • PCR, real-time PCR, qPCR
  • HLA Typing
  • Southern Blot

Manual

MSDS

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  • Product: INVIMAG UNIVERSAL KIT/ STARLET
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