Overview

The InviMag® Virus RNA Kit/ KFmL allows for rapid and economical purification of viral RNA from up to 200 µl of clinical samples using magnetic beads. The kit is designed for semi-automated preparation on the KingFisher mL workstation.

Benefits

• Linear yields - efficient purification over a wide range of viral titers

• Standardized processing ensures reproducible results

• For In Vitro Diagnostic Use (CE-IVD)*

Product Characteristics

The kits are also available without the KingFisher plastic

Workflow

The sample is lysed in an optimized lysis buffer containing Proteinase K and Carrier RNA. The addition of Carrier RNA is necessary for the enhancement of RNA recovery in samples with low virus titer. Carrier RNA also stabilizes nucleic acids in samples with very small nucleic acid concentrations. The cleared lysates are transferred to the automatic purification procedure. The viral RNA binds to the magnetic beads, followed by washing steps and the final elution. Manual handling of potentially infectious samples is minimized, ensuring safety to the user and reliable processing of samples. The isolated viral RNA is ready to use for subsequent downstream applications:

• RNA dot blots

• cDNA transcription

• Real-time PCR (quantitative RT-PCR, like TaqMan and LightCycler technologies)

• Array technologies

*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.

Manual

MSDS