Overview

The InviQuant GeneCount 40 is a qPCR-based system to determine the quantity of human genomic DNA e.g. prior to use in NGS or other downstream applications. Unlike other approaches that target a single genomic locus, the InviQuant GeneCount 40 uses a qPCR assay to detect 40 genomic loci which are randomly distributed throughout the human genome. The proprietary design ensures minimal variation caused by local genomic events. The use of the included high-quality DNA standard allows quantification of amplifiable targets for example in cell-free circulating DNA (cfDNA) samples.

Benefits

• Provides qPCR based quantification of up to 200 samples

• Quantifies traces of human genomic DNA even, highly fragmented DNA such as cfDNA or DNA from FFPE or forensic samples

• Suitable for determining the correct amount of input DNA for NGS applications

• Increased sensitivity and reliability in comparison to single copy qPCR through detection of 40 randomly distributed genomic loci

Product Characteristics

Workflow

The assay contains an internal amplification control for the determination of PCR inhibitors, as well as a standard DNA for preparing a dilution series with defined copy numbers. The use of a Standard DNA Dilutions Series allows quantification of amplifiable molecules in gDNA samples.

The workflow for the InviQuant GeneCount 40 assay is simple and convenient. After extracting gDNA from biological samples simply add the DNA to the qPCR Master-Mix. Real-time PCR is performed according to manufacturer’s recommendations and CT values are exported to calculate the concentration of the sample by using the linear equation generated with the Standard DNA Dilutions Series.

Application

Manual

MSDS