INVISORB BLOOD MINI HTS 96 KIT
Overview
The Invisorb® Blood Mini HTS 96 Kit is designed for genomic DNA purification from human and animal blood as well from buffy coat or bone marrow in 96 well format. Fresh or frozen whole blood treated with EDTA or citrate, but not with heparin, from common blood collection systems can be used.
Benefits
- No additional erythrocyte lysis
- Reproducible high yields
- For In Vitro Diagnostic Use (CE-IVD)*
Product Characteristics
|
Starting Material |
Average Yield |
Preparation Time |
|
up to 200 µl human blood
up 75 µl mammalian blood |
up to 10 µg (in average 6 µg) |
about 25 min |
Workflow
The purification procedure is performed in a deep-well centrifuge. The whole blood sample is lysed in an optimized lysis buffer and proteins are degraded during lysis with Proteinase K. Genomic DNA is bound on the surface of the membrane of the filter plate. All impurities are removed by washing steps and the DNA is eluted in a low-salt buffer. Due to the high purity the isolated genomic DNA is ready to use for subsequent downstream applications:
- PCR
- real-time PCR
- RFLP analysis
- restriction enzyme digestion
- hybridization
- SNP analysis
- HLA typing
- genotyping
*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.
- Product: INVISORB BLOOD MINI HTS 96 KIT
- SKU:
- Availability: In Stock