Overview

Use the Invisorb® Spin Virus RNA Mini Kit for reliable isolation of high-quality RNA from viruses found in a diverse range of starting materials. The kit simplifies viral RNA isolation by combining efficient lysis of the starting material and the inactivation of exogenous and endogenous RNases to prevent degradation.

Benefits

• RT-PCR templates in 25 minutes with high sensitivity at low virus titers

• Safe handling of infectious samples by using capped spin columns

• For In Vitro Diagnostic Use (CE-IVD)*

Product Characteristics

Workflow

The sample is lysed in an optimized lysis buffer and proteins are degraded during the lyses with Proteinase K. To guarantee RNA integrity, samples are lysed under highly denaturing conditions to inactivate RNases. The addition of Carrier RNA is necessary for the enhancement of DNA recovery in samples with low virus titer. Carrier RNA also stabilizes nucleic acids in samples with very small nucleic acid concentrations. The liberated RNA is bound onto the membrane of the Spin Filter. Contaminants are removed by repeated washing steps and the purified viral RNA can be eluted in a small volume of RNase-free water. Due to the high purity, the isolated viral RNA is ready to use for a broad panel of downstream applications like:

• RT-PCR

• Taq Man analysis and array technologies

• RNA dot blots

• cDNA transcription

• in vitro translation

*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.

Application

Manual

MSDS

References