Overview

The Invisorb® Universal HTS 96 Kit/ STARlet is designed for fully automated extraction and purification of viral DNA/ RNA, genomic DNA, bacterial DNA from 96 samples per run with the STARlet system from Hamilton.

Benefits

• One kit for a variety of starting materials and isolation of different nucleic acids under uniform conditions
• Automated, walk-away DNA purification of 96 samples
• High reproducibility through standardized processing

Product Characteristics

Workflow

The STARlet instrument uses an 8 channel pipettor to transfer samples and buffers and a vacuum station to separate the DNA/ RNA during the various extraction phases: lysis-binding-washing-elution. The automated purification process results in a fast, reliable and robust technique.

After a sample specific lysis using Lysis Buffer HLT and Proteinase K (Lysozyme too if required - not provided), binding conditions are adjusted upon addition of Binding Solution. The genomic DNA and/or viral DNA/ RNA bind to DNA Binding Plate B and is separated from the solution by the vacuum pump controlled by the STARlet system. Subsequent to three washing steps of the bound nucleic acids, the nucleic acids are finally eluted in Elution Buffer M. 

Due to the high purity the eluted nucleic acids are ready-to-use use for subsequent downstream applications:

• PCR, real-time PCR, qPCR

• HLA Typing

• Southern Blot

Manual

MSDS