INVISORB VIRUS DNA HTS 96 KIT
Overview
The Invisorb® Virus DNA HTS 96 Kit is designed for reliable and fast purification of viral DNA from clinical samples in 96 well format, optimized for use in a centrifuge.
Benefits
- Sensitive and accurate results at low viral titers
- No cross contamination
- For In Vitro Diagnostic Use (CE-IVD)*
Product Characteristics
|
Starting Material |
Average Yield |
Preparation Time |
|
up to 200 µl plasma, serum, whole blood, cell-free body fluids of
human origin, rinse liquid from swabs |
depending
on viral titer |
about 30 min after lysis |
Workflow
The sample is lysed in an optimized lysis buffer containing a carrier nucleic acid. The addition of Carrier RNA is necessary for the enhancement of DNA recovery in samples with low virus titer. Carrier RNA also stabilizes nucleic acids in samples with very small nucleic acid concentrations. The viral proteins are degraded during lysis with Proteinase K at higher temperatures. The DNA binds to the filter membrane, followed by washing steps and the final elution. The procedure requires minimal interaction from the user, allowing safe handling of infectious samples. The procedures are designed to avoid sample-to-sample cross contamination. The isolated viral DNA is ready to use for subsequent downstream applications:
- PCR
- Real-time PCR (quantitative PCR, like TaqMan and LightCycler technologies)
- Restriction enzyme digestion
*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.
- Product: INVISORB VIRUS DNA HTS 96 KIT
- SKU:
- Availability: In Stock