INVISORB VIRUS RNA HTS 96 KIT
Overview
The Invisorb® Virus RNA HTS 96 Kit is designed for reliable and fast purification of viral RNA from clinical samples in 96 well format, optimized for use in a centrifuge.
Benefits
- Sensitive and accurate results at low viral titers
- No cross contamination
- For In Vitro Diagnostic Use (CE-IVD)*
Product Characteristics
|
Starting Material |
Average Yield |
Preparation Time |
|
up to 200 µl plasma, serum, cell-free body fluids of human origin,
rinse liquid from swabs up to 50 µg stool samples |
depending
on viral titer |
approx. 70 min |
Workflow
The sample is lysed in an optimized lysis buffer containing carrier nucleic acids. Viral proteins are degraded during lysis with Proteinase K. The addition of Carrier RNA is necessary for the enhancement of RNA recovery in samples with low viral titer. Carrier RNA also stabilizes nucleic acids in samples with very small nucleic acid concentrations. The viral RNA binds to the filter membrane, followed by washing steps and the final elution. The procedure requires minimal interaction by the user, allowing safe handling of infectious samples. The procedures are designed to avoid sample-to-sample cross contamination. Due to the high purity the isolated viral RNA is ready to use for downstream applications or can be stored at – 80°C for subsequent use:
- RT-PCR
- real-time RT-PCR (quantitative RT-PCR, like TaqMan and LightCycler technologies)
*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.
- Product: INVISORB VIRUS RNA HTS 96 KIT
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- Availability: In Stock