Iam BCR-ABL Kit

Background

The BCR-ABL fusion gene codes for an oncogenic protein with elevated tyrosine kinase activity, which is responsible for the neoplastic transformation. The timely and accurate molecular detection of the BCR-ABL transcripts is mandatory in order to diagnose Philadelphia Positive Leukemias allowing implementation of targeted therapies, which are able to selectively inactivate the BCR-ABL chimeric protein.

Why to choose it

A simplified, comprehensive and rapid solution for the reliable molecular identification of BCR-ABL translocations.

Benefits

Enhanced formulation

Comprehensive detection of common and rare BCR-ABL isoforms

Clinical studies performed on the limited number of available samples (n=14) have demonstrated that the assay is capable of detecting the rarer isoforms of p190 and p210, and the p230 variant (μbcr).

Ultra Rapid

Identification of the translocation within 20 minutes, starting form extracted RNA

One step, closed format

The assay works directly on 500 ng RNA with no need for multistep procedures: decreased risk of contamination and errors

Highly Specific 

No false positive results and absence of primer dimers

Tested on 521 BCR-ABL negative cell lines RNA extracted by both Qiagen Rneasy kit and modified TRIzol and on 225 NTC, the assay shows >99.8% specificity

Highly Sensitive

One traslocated RNA in 1000 wild type is reproducible detected (>95% of cases).

K562 and TOM-1 10-4 dilutions were detected in ≥ 95% of replicates; K562 10-4 / TOM-1 10-4 dilutions extracted with Qiagen RNeasy Kit and modified TRIzolTM were detected 87.5%/90% and 88.4%/85% respectively.

EXTRACTION METHOD

Clinically Validated

One traslocated RNA in 1000 wild type is reproducible detected (>95% of cases)

Robust

Superior robustness to inhibitors, RNA degradation and suboptimal target quantities.

The intrinsic robustness of the enzyme employed in Q-LAMP results in a enhanced robustness versus the classical inhibitor factors of PCR.

Superiority vs. PCR

Q-LAMP is a very rapid, easy, accurate, convenient and robust method for detection of BCR-ABL translocations.

Can be implemented even in not specialized laboratories for a timely Philadelphia Positive Leukemias diagnosis and as a screening for exclusion of Philadelphia Positive conditions.

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