Overview

The RTP® Bacteria DNA Mini Kit contains the pre-filled Extraction Tubes with lyophilized lysis components for genomic DNA isolation from different bacteria species from a broad range of starting materials. After lysis, the samples are transferred to a spin column-based procedure with a PCR template preparation time of 25 minutes. The „One-Step-Lysis“ simplifies handling and reduces pipetting steps.

Benefits

• Simple „One-Step-Lysis“ in the pre-filled Extraction Tube with lyophilized lysis components (lysis buffer, Lysozyme, Proteinase K, Carrier RNA); Extraction Tubes can be stored at room temperature

• Sensitive and accurate results

• For In Vitro Diagnostic Use (CE-IVD)*

Product Characteristics

Workflow

Using the RTP Bacteria DNA Mini Kit the sample is transferred to the Extraction Tube together with a specially designed Resuspension Buffer. After cell wall lysis of bacterial cells using Lysozym provided in the Extraction Tube, the pre-filled buffer and Proteinase K lyse the material and digest the proteins and stabilize the nucleic acid by inactivating intracellular DNases. This is followed by the addition of a binding buffer to enhance selective DNA adsorption to the membranes. The membranes are efficiently washed before the DNA is eluted to remove all contaminants. The purified high quality DNA is ready to use for subsequent downstream applications or can be stored at –20 °C for subsequent use:

• PCR

• real-time PCR

• RFLP analysis

*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized

Application

Manual

MSDS

References