RTP DNA/RNA VIRUS MINI KIT

  • RTP DNA/RNA VIRUS MINI KIT

Overview

The RTP® DNA/RNA Virus Mini Kit contains the pre-filled Extraction Tubes with lyophilized lysis components for viral DNA and RNA isolation from a diverse range of starting materials. After lysis the samples are transferred to a spin column-based procedure with a total preparation time of PCR/ RT-PCR templates in just 25 minutes.

Benefits

  • Simple „One-Step-Lysis“ in the pre-filled Extraction Tube with lyophilized lysis components (lysis buffer, Proteinase K, Carrier RNA); Extraction Tubes can be stored at room temperature
  • Sensitive and accurate results at low viral titers
  • For In Vitro Diagnostic Use (CE-IVD)*
  • One kit for both DNA and RNA viral purification allowing simultaneous testing of both virus types

Product Characteristics

Starting Material

Average Yield

Preparation Time

up to 200 μl serum, plasma, cell-free body fluids, cell culture supernatant

up to 400 µl rinse liquid from swabs
up to 10 mg tissue samples
stool suspension

depending on viral titer

about 25 min

Workflow

Using the RTP DNA/RNA Virus Mini Kit the sample is lysed in the pre-filled Extraction Tube containing lyophilized lysis reagents and carrier nucleic acid. The lysis reagents further stabilize the viral nucleic acids, inactivate RNases and DNases and enhance the selective DNA /RNA adsorption to the membrane of the Spin Filter. Before the viral nucleic acids are eluted, the membranes are efficiently washed in order to completely remove all PCR inhibitors.

*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.

Application

Manual

MSDS

References

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  • Product: RTP DNA/RNA VIRUS MINI KIT
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