Overview

The RTP® Mycobacteria Kit contains the pre-filled Extraction Tubes with lyophilized lysis components for DNA isolation from Mycobacteria species from sputum, bronchoalveolar lavage and small tissue samples. After lysis, the samples are transferred to a spin column-based procedure. The „One-Step-Lysis“ simplifies handling and reduces pipetting steps.

Benefits

• Simple „One-Step-Lysis“ in the pre-filled Extraction Tube with lyophilized lysis components (lysis buffer, Lysozyme, Proteinase K, Carrier RNA); Extraction Tubes can be stored at room temperature

• Special buffer for reduction of the sample viscosity is provided with the kit

• For In Vitro Diagnostic Use (CE-IVD)*

Product Characteristics

Workflow

The kit includes NAC Buffer for the standard pretreatment of 200 μl – 5 ml sputum samples before lysis of the material. The resulting bacterial cell pellet is dissolved in a special Resuspension Buffer and completely transferred to the Extraction Tube. Optimized buffer and enzymes lyse the samples under a special temperature profile, stabilize nucleic acids and selectively enhance DNA adsorption to the membrane. The membranes are efficiently washed before the nucleic acids are eluted in order to remove all PCR inhibitors and proteins. Based on patented technology, the kit uses buffers without any hazardous chaotropic components. The purified DNA is ready to use for subsequent downstream applications or can be stored at –20 °C:

• PCR

• real-time PCR (quantitative PCR, like TaqMan and LightCycler technologies)

• restriction digestion

• RFLP analysis

*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized

Application

Manual

MSDS