Overview

The RTP® Pathogen Kit contains the pre-filled Extraction Tubes with lyophilized lysis components for simultaneous isolation of high quality bacterial DNA, viral DNA and RNA from a diverse range of starting materials. After lysis, the samples are transferred to a spin column-based procedure with a PCR template preparation time of 25 minutes. The „One-Step-Lysis“ simplifies handling and reduces pipetting steps.

Benefits

• Simple „One-Step-Lysis“ in the pre-filled Extraction Tube with lyophilized lysis components (lysis buffer, Lysozyme, Proteinase K, Carrier RNA); Extraction Tubes can be stored at room temperature
• Sensitive and accurate results
• For In Vitro Diagnostic Use (CE-IVD)*

Product Characteristics

Workflow

Using the RTP Pathogen Kit all types of samples are transferred to the Extraction Tube together with a specially designed Resuspension Buffer. The pre-filled buffer and enzymes lyse the samples, stabilize the nucleic acids, inactivate RNases and DNases and enhance the viral and bacterial DNA and/ or RNA adsorption to the membrane of the Spin Filter. Contaminants are removed by repeated wash steps and the purified nucleic acids can be eluted in a small volume of Elution Buffer.

*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.

Manual

MSDS

References