Invisorb Spin Tissue Mini Kit

    Overview

The Invisorb® Spin Tissue Mini Kit simplifies genomic DNA purification from a variety of tissue types (e.g. muscle, liver, heart, brain, mouse tail or insects) and cells. PCR inhibitors, which can interfere with the amplification reaction, are efficiently removed. Pure DNA is eluted in 50 – 200 µl EDTA-free buffer or water and can be used in subsequent downstream applications, such as PCR, real-time PCR, SNP detection, Southern Blotting, sequencing and cloning.

    Benefits

• Optimized protocols for a variety of starting materials, complete removal of contaminants and inhibitors

• More intact DNA: no DNA degradation by using a gentle, low-salt (non-chaotropic) buffer system

• For In Vitro Diagnostic Use (CE-IVD)*

Product Characteristics

   Workflow

The Invisorb Spin Tissue Mini Kit uses a special buffer and Proteinase S for efficient lysis of the tissue samples. After a centrifugation step the precleaned lysate is mixed with the binding buffer and transferred onto the spin filter column. The DNA binds to the membrane, impurities are washed away and reproducible ultra-pure DNA is eluted. The purified DNA is ready to use for subsequent downstream applications or can be stored at –20 °C for subsequent use:

• PCR

• real-time PCR

• sequencing

• cloning

• RFLP analysis

• restriction enzyme digestion

• hybridization

• SNP detection

*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized

User Manual