INVITRAP RNA PLANT HTS 96 KIT

  • INVITRAP RNA PLANT HTS 96 KIT

Overview

The InviTrap® RNA Plant HTS 96 Kit is a powerful tool for purification of total RNA from up to 50 mg of plant tissue in 96 well format using a centrifuge.

Benefits

  • Pure RNA without DNase digestion - selective genomic DNA removal during lysis step
  • Two sample-specific lysis buffers included - contaminants, such as polysaccharides, or polyphenols are removed
  • No cross contamination

Product Characteristics

Starting Material

Average Yield

Preparation Time

up to 50 mg homogenized plant tissue

up to 30 µg

approx. 60 min

Workflow

During sample lysis the genomic DNA selectively binds to carrier particles and is removed by a pre-filtration step, clearing simultaneously the lysate from the unlysed plant material. A DNase digestion step is not necessary. After DNA removal and adjustment of binding conditions the solution is transferred to the RNA-Binding Plate. The RNA binds to the membrane and contaminants are efficiently washed away. The isolated total RNA is ready to use for downstream applications:

  • RT-PCR
  • real-time RT-PCR
  • Northern Blot analysis
  • microarray analysis

Application

Manual

MSDS

References 

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  • Product: INVITRAP RNA PLANT HTS 96 KIT
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