INVITRAP SPIN UNIVERSAL RNA MINI KIT

  • INVITRAP SPIN UNIVERSAL RNA MINI KIT

Overview

The InviTrap® Spin Universal RNA Mini Kit simplifies total RNA isolation from different starting materials such as cells, tissue and blood. The genomic DNA is removed without an enzymatic digestion step. RNases are inactivated to prevent RNA degradation. Furthermore all InviTrap RNA kits feature capped spin columns for safe and reliable RNA purification.

Benefits

  • Pure RNA without DNase digestion - selective genomic DNA removal during lysis step
  • Optimized protocols for cells, tissue and blood - incl. additional protocols for RNA Cleanup from TRIzol® phases and enzymatic reactions, protein/RNA extraction, RNA/DNA isolation
  • For In Vitro Diagnostic Use (CE-IVD)*

Product Characteristics

Starting Material

Average Yield

Preparation Time

- up to 107 cells

- up to 20 mg tissue
- up to 1.5 ml whole blood

up to 100 µg total RNA

up to 80 µg total RNA
up to 1 - 5 µg total RNA / ml of whole blood

10 – 45 min

 


Workflow

The kit simplifies total RNA isolation by combining efficient lysis of the starting material and the inactivation of exogenous and endogenous RNases with the efficient removal of genomic DNA by selectively binding to a specific surface of a solid material. Subsequently, the RNA is bound onto the membrane of the Spin Filter. Contaminants are removed by following washing steps and highly purified RNA can be eluted in small volume of RNase free water. Due to the high purity, the isolated total RNA is ready to use for subsequent downstream applications like:

    • RT-PCR
    • RNA dot blots
    • cDNA-library
    • in vitro translation
    • DDRT-PCR
    • TaqMan analysis and array technologies

    *) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized. Not valid for supplemental protocols.

    Application

    Manual

    MSDS

    References

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    • Product: INVITRAP SPIN UNIVERSAL RNA MINI KIT
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