INVISORB FRAGMENT CLEANUP
Overview
The Invisorb® Fragment CleanUp provides a convenient tool for fast and efficient purification of PCR products and DNA fragments from amplification or enzymatic reactions as well as for fast and efficient extraction of DNA fragments from agarose gels.
Benefits
- PCR cleanup ready in 7 minutes - only binding and elution
- Elution volume as little as 10 μl, also suitable for sample concentration
- Dye removal from Cycle-Sequencing reactions
- Multiple applications in one kit incl. gel extraction
Product Characteristics
|
Starting Material |
Average
Yield |
Preparation Time |
|
up to 100 ml PCR-,
Cycle Sequencing- cDNA synthesis-, ligation- and restriction digestion
reaction mixture up to 300 mg gel slices from TAE
or TBE agarose gels |
up to 95 %, depending on
fragment length and kind of agarose gel |
7 min for PCR
cleanup
20 min for gel extraction |
Workflow
For PCR or DNA fragment cleanup the MSB technology is used, i.e. washing steps are not necessary. After adjustment of binding conditions the DNA fragments bind to the membrane of the spin filter. The flow-through contains all contaminants. Finally, the DNA fragments are eluted in a low-salt buffer or water. For purification of DNA fragments from agarose gels, the DNA fragments are bound to the surface of the spin filter column after the gel is solubilized. The purified DNA fragments are ready to use in various downstream applications:
- sequencing
- cloning
- ligation
- labelling
- digestion with restriction enzymes
- PCR
- Product: INVISORB FRAGMENT CLEANUP
- SKU:
- Availability: In Stock