INVISORB SPIN VIRUS DNA MINI KIT
Overview
Use the Invisorb® Spin Virus DNA Mini Kit for reliable isolation of high-quality DNA from viruses found in a diverse range of starting materials. Fresh or frozen whole blood treated with EDTA or citrate, but not with heparin, from common blood collection systems can be used.
Benefits
- Safe handling of infectious samples by using capped spin columns
- Sensitive and accurate results at low viral titers
- For In Vitro Diagnostic Use (CE-IVD)*
Product Characteristics
|
Starting Material |
Average Yield |
Preparation Time |
|
up to 200 µl mammalian blood, plasma, serum, CSF, rinse liquid from
swabs, cell culture supernatant
up to 25 µl non-mammalian blood |
depending
on vital titer |
25 min |
Workflow
The sample is lysed in an optimized lysis buffer containing Carrier RNA and proteins which are degraded during lysis with Proteinase K. The addition of Carrier RNA is necessary for the enhancement of DNA recovery in samples with low virus titer. Carrier RNA also stabilizes nucleic acids in samples with very small nucleic acid concentrations. The liberated DNA is bound onto the membrane of the RTA Spin Filter. Contaminants are removed by repeated washing steps and the purified viral DNA is finally eluted. Due to the high purity the isolated viral DNA is ready to use for downstream applications like:
- PCR
- real-time PCR (quantitative PCR, like TaqMan and LightCycler technologies)
- restriction enzyme digestion.
*) Compliance with EU Directive 98/79/EC on in vitro medical devices. Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.
- Product: INVISORB SPIN VIRUS DNA MINI KIT
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- Availability: In Stock