Iam PML-RARA Kit

Iam PML-RARA Kit

Background

The PML-RARA t (15;17) translocation is associated with the Acute Promyelocytic Leukemia (APL), a potentially fatal subtype of Acute Myeloid Leukemia due to severe coagulopathy. Accuracy and speed of the diagnostic work-up are critical to allow timely initiation of efficient therapies that can convert APL from a highly fatal into a highly curable Leukemia. Iam PML-RARA detection bcr1,3 kit and Iam PML-RARA discrimination bcr2 kit are to be used in combination for accurate identification of bcr1/bcr2/bcr3 isoforms (100% specificity).

Why to choose it

A simplified, reliable and rapid solution: APL molecular diagnosis within 20 minutes is now possible

Benefits

Ultra Rapid

The assay identifies a bcr1/bcr2/bcr3 positivity with an average threshold time of 16,52 ± 3,33 min. Amplification curves are visible in real time, to allow preliminary identification of positive urgent samples.

One step, closed format

The assay works directly on 500 ng RNA with no need for multistep procedures: decreased risk of contamination and errors

100% Specific 

No false positive results and absence of primer dimers

Tested on 400 PML-RARA negative cell lines RNA, extracted by both Qiagen Rneasy kit and modified TRIzol, and on 200 NTC, the assay do not generate any aspecific signal.

PML-RARA DETECTION BCR1,3

RNA was extracted from PML-RARA negative cell lnes by both Qiagen RNeasy kit and modified TRizol.

PML-RARA DISCRIMINATION BCR2

Sample type	Replicates	% Analytical Specificity
Cell lines	400	100%
NTC	210	100%
Total replicates	610	100%

RNA was extracted from PML-RARA negative cell lines by both Qiagen RNeasy kit and modified TRizol

Internal endogenous control and quality controls are included to allow validation of results

Clinically Validated

All clinical analysis has been performed on RNA extracted by both Qiagen RNeasy and modified TRIzol.

All clinical analysis has been performed on RNA extracted by both Qiagen RNeasy and modified TRIzol

Robust

Superior robustness to inhibitors, RNA degradation and suboptimal target quantities

The intrinsic robustness of the enzyme employed in Q-LAMP results in a enhanced robustness versus the classical inhibitor factors of PCR.

Superior Performance

Q-LAMP is a very rapid, easy, accurate, convenient and robust method for detection of AML1-ETO translocations.

It can be implemented even in not specialized laboratories for a timely effective APL diagnosis, which is key for initiation of therapy.

For use on

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Ordering Info

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BROCHURE PML-RARA