INVIMAG STOOL DNA MINI KIT/ KFML
-800x800.png "INVIMAG STOOL DNA MINI KIT/ KFML")
-800x800.png "INVIMAG STOOL DNA MINI KIT/ KFML")
-800x800.png "INVIMAG STOOL DNA MINI KIT/ KFML")
Overview
The InviMag® Stool DNA Mini Kit/ KFmL allows for rapid and economical purification of genomic and microbial DNA from up to 200 mg of stool sample or about 1 g sample in 8 ml Stool Stabilizer using magnetic beads. The kit is designed for semi-automated preparation on the KingFisher mL workstation.
Benefits
- Complete removal of PCR inhibitors
- Lysis conditions are optimized for isolation of microbial and cellular DNA from animal or human stool
- Samples can be stored in the Stool Collection Tubes, prefilled with Stool DNA Stabilizer - Samples are stable at room temperature for 3 months for easy transport and storage
Product Characteristics
|
Starting Material |
Average Yield |
Preparation Time |
|
200 mg or
about
1 g sample in 8 ml |
up to 50 µg |
about 20 -25 min after lysis |
The kits are also available without the KingFisher plastic.
Workflow
A rigorous pre-lysis step with optimized pre-lysis buffer under high temperatures is followed by a pre-incubation of the sample with InviAdsorb to remove PCR inhibitors outside of the robotic platform. Undissolved particles and PCR inhibitors bound to InviAdsorb are removed by a centrifugation step. Besides the pre-filling of all cavities, all following processing steps are performed by the robot, like Proteinase K digestion and the binding of DNA to magnetic particles, followed by washing steps and the final elution. The choice of different lysis conditions allow for the enrichment or a reduction of the content of bacterial DNA in the total DNA in favor of human DNA.
The purified high quality DNA is ready to use for subsequent downstream applications or can be stored at –20 °C for subsequent use:
- PCR applications
- hybridization
- genetic typing
- pathogen typing
- mutation analysis
- Product: INVIMAG STOOL DNA MINI KIT/ KFML
- SKU:
- Availability: In Stock